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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene is observed in several tumors, which results in chromosome instability, and therefore may perhaps lead to tumorigenesis. This protein was identified to affiliate with, and become involved in, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in cell-cycle regulated histone gene expression and performs a vital purpose in promoting mobile-cycle progression within the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, happen to be explained. Two added splice variants had been described but thorough nucleotide sequence info will not be but available.
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Blocking peptides are peptides that bind precisely for the concentrate on antibody and block antibody binding. These peptide generally is made up of the epitope regarded from the antibody.
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This guidebook outlines additional feasible optimization of mobile and immunoassay parameters to guarantee the absolute best outcomes are...
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This guide outlines additional achievable optimization of cellular and immunoassay parameters to make certain the absolute best final results are...
Antibodies certain to the blocking peptide not bind for the epitope about the goal protein. By comparing the staining in the blocked antibody versus the antibody alone, you can see which staining is unique.
Focus on Facts Cyclin E belongs on the hugely conserved cyclin loved ones, whose members are characterised by a extraordinary periodicity in protein abundance throughout the cell cycle. Cyclins operate as regulators of CDK kinases. Various cyclins show distinctive expression and degradation patterns which contribute to the temporal coordination of each and every mitotic function. Cyclin E types a posh with and features like a regulatory subunit of CDK2, whose activity is needed for cell cycle G1/S changeover. This protein accumulates on the G1-S phase boundary and is particularly degraded as cells progress through S period.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by each antibodies...
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